truncated pet-22b (+) expression vector (Millipore)
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Millipore
truncated pet-22b (+) expression vector
Truncated Pet 22b (+) Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet-22b(+)+vector/pet22b/bio_rxiv__2024__06__29__601316-56-12-11
Average 90 stars, based on 1 article reviews
Truncated Pet 22b (+) Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet-22b(+)+vector/pet22b/bio_rxiv__2024__06__29__601316-56-12-11
Average 90 stars, based on 1 article reviews
truncated pet-22b (+) expression vector - by Bioz Stars,
2026-10
90/100 stars
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Clone Assay:Article Title: Characterization of a Novel Esterase Est33 From an Antarctic Bacterium: A Representative of a New Esterase Family Article Snippet: The purified PCR product was ligated into the pET-22b vector (Novagen, United States) with Hind III and Nde I using NovoRec® plus One-step PCR Cloning Kit (Novoprotein, China). Article Title: Ubiquitous occurrence of a dimethylsulfoniopropionate ABC transporter in abundant marine bacteria Article Snippet: The related genes were then cloned into the Nde I/ Xho I restriction sites of the pET-22b vector (Novagen, Germany) with a C-terminal His tag. Article Title: TERRA R-loops connect and protect sister telomeres in mitosis Article Snippet: For expression in E. coli, C19orf43 was cloned into the pET-22b Vector (Novagen) with a C-terminal His tag and expressed without the pelB leader sequence. Article Title: Ligand discovery for T cell receptors Article Snippet: The α and β chains of Ob.1A12 and Ob.2F3 TCRs were separately cloned into the Article Title: TERRA R-loops connect and protect sister telomeres in mitosis. Article Snippet: For expression in E. coli, C19orf43 was cloned into the pET-22b Vector (Novagen) with a C-terminal His tag and expressed without the pelB leader sequence. Article Title: Crystal structure and biochemical analysis suggest that YjoB ATPase is a substrate-specific molecular chaperone Article Snippet: To prepare GroEL, DNA encoding B. subtilis GroEL (residues 1-544) was inserted into the Article Title: SAR92 clade bacteria are potentially important DMSP degraders and sources of climate-active gases in marine environments Article Snippet: The amplified genes were digested and cloned into the Nde I/ Xho I restriction sites of the pET-22b vector (Novagen, Germany) to incorporate a C-terminal His tag. Article Title: Periplasmic Expression of a Novel Human Bone Morphogenetic Protein-7 Mutant in Escherichia coli Article Snippet: The fragment encoding the genetically modified mature part was successfully cloned into the NdeI/EcoRІ site of pET-22b vector (Novagen, USA). Plasmid Preparation:Article Title: Characterization of a Novel Esterase Est33 From an Antarctic Bacterium: A Representative of a New Esterase Family Article Snippet: The purified PCR product was ligated into the pET-22b vector (Novagen, United States) with Hind III and Nde I using NovoRec® plus One-step PCR Cloning Kit (Novoprotein, China). Article Title: Ubiquitous occurrence of a dimethylsulfoniopropionate ABC transporter in abundant marine bacteria Article Snippet: The related genes were then cloned into the Nde I/ Xho I restriction sites of the pET-22b vector (Novagen, Germany) with a C-terminal His tag. Article Title: TERRA R-loops connect and protect sister telomeres in mitosis Article Snippet: For expression in E. coli, C19orf43 was cloned into the pET-22b Vector (Novagen) with a C-terminal His tag and expressed without the pelB leader sequence. Article Title: Ligand discovery for T cell receptors Article Snippet: The α and β chains of Ob.1A12 and Ob.2F3 TCRs were separately cloned into the Article Title: TERRA R-loops connect and protect sister telomeres in mitosis. Article Snippet: For expression in E. coli, C19orf43 was cloned into the pET-22b Vector (Novagen) with a C-terminal His tag and expressed without the pelB leader sequence. Article Title: Crystal structure and biochemical analysis suggest that YjoB ATPase is a substrate-specific molecular chaperone Article Snippet: To prepare GroEL, DNA encoding B. subtilis GroEL (residues 1-544) was inserted into the Article Title: SAR92 clade bacteria are potentially important DMSP degraders and sources of climate-active gases in marine environments Article Snippet: The amplified genes were digested and cloned into the Nde I/ Xho I restriction sites of the pET-22b vector (Novagen, Germany) to incorporate a C-terminal His tag. Article Title: Periplasmic Expression of a Novel Human Bone Morphogenetic Protein-7 Mutant in Escherichia coli Article Snippet: The fragment encoding the genetically modified mature part was successfully cloned into the NdeI/EcoRІ site of pET-22b vector (Novagen, USA). Expressing:Article Title: Characterization of a Novel Esterase Est33 From an Antarctic Bacterium: A Representative of a New Esterase Family Article Snippet: The purified PCR product was ligated into the pET-22b vector (Novagen, United States) with Hind III and Nde I using NovoRec® plus One-step PCR Cloning Kit (Novoprotein, China). Article Title: Ubiquitous occurrence of a dimethylsulfoniopropionate ABC transporter in abundant marine bacteria Article Snippet: The related genes were then cloned into the Nde I/ Xho I restriction sites of the pET-22b vector (Novagen, Germany) with a C-terminal His tag. Article Title: TERRA R-loops connect and protect sister telomeres in mitosis Article Snippet: For expression in E. coli, C19orf43 was cloned into the pET-22b Vector (Novagen) with a C-terminal His tag and expressed without the pelB leader sequence. Article Title: Ligand discovery for T cell receptors Article Snippet: The α and β chains of Ob.1A12 and Ob.2F3 TCRs were separately cloned into the Article Title: TERRA R-loops connect and protect sister telomeres in mitosis. Article Snippet: For expression in E. coli, C19orf43 was cloned into the pET-22b Vector (Novagen) with a C-terminal His tag and expressed without the pelB leader sequence. Article Title: Crystal structure and biochemical analysis suggest that YjoB ATPase is a substrate-specific molecular chaperone Article Snippet: To prepare GroEL, DNA encoding B. subtilis GroEL (residues 1-544) was inserted into the Article Title: SAR92 clade bacteria are potentially important DMSP degraders and sources of climate-active gases in marine environments Article Snippet: The amplified genes were digested and cloned into the Nde I/ Xho I restriction sites of the pET-22b vector (Novagen, Germany) to incorporate a C-terminal His tag. Article Title: Periplasmic Expression of a Novel Human Bone Morphogenetic Protein-7 Mutant in Escherichia coli Article Snippet: The fragment encoding the genetically modified mature part was successfully cloned into the NdeI/EcoRІ site of pET-22b vector (Novagen, USA). Sequencing:Article Title: Characterization of a Novel Esterase Est33 From an Antarctic Bacterium: A Representative of a New Esterase Family Article Snippet: The purified PCR product was ligated into the pET-22b vector (Novagen, United States) with Hind III and Nde I using NovoRec® plus One-step PCR Cloning Kit (Novoprotein, China). Article Title: Ubiquitous occurrence of a dimethylsulfoniopropionate ABC transporter in abundant marine bacteria Article Snippet: The related genes were then cloned into the Nde I/ Xho I restriction sites of the pET-22b vector (Novagen, Germany) with a C-terminal His tag. Article Title: TERRA R-loops connect and protect sister telomeres in mitosis Article Snippet: For expression in E. coli, C19orf43 was cloned into the pET-22b Vector (Novagen) with a C-terminal His tag and expressed without the pelB leader sequence. Article Title: Ligand discovery for T cell receptors Article Snippet: The α and β chains of Ob.1A12 and Ob.2F3 TCRs were separately cloned into the Article Title: TERRA R-loops connect and protect sister telomeres in mitosis. Article Snippet: For expression in E. coli, C19orf43 was cloned into the pET-22b Vector (Novagen) with a C-terminal His tag and expressed without the pelB leader sequence. Article Title: Crystal structure and biochemical analysis suggest that YjoB ATPase is a substrate-specific molecular chaperone Article Snippet: To prepare GroEL, DNA encoding B. subtilis GroEL (residues 1-544) was inserted into the Article Title: SAR92 clade bacteria are potentially important DMSP degraders and sources of climate-active gases in marine environments Article Snippet: The amplified genes were digested and cloned into the Nde I/ Xho I restriction sites of the pET-22b vector (Novagen, Germany) to incorporate a C-terminal His tag. Article Title: Periplasmic Expression of a Novel Human Bone Morphogenetic Protein-7 Mutant in Escherichia coli Article Snippet: The fragment encoding the genetically modified mature part was successfully cloned into the NdeI/EcoRІ site of pET-22b vector (Novagen, USA). Amplification:Article Title: Characterization of a Novel Esterase Est33 From an Antarctic Bacterium: A Representative of a New Esterase Family Article Snippet: The purified PCR product was ligated into the pET-22b vector (Novagen, United States) with Hind III and Nde I using NovoRec® plus One-step PCR Cloning Kit (Novoprotein, China). Article Title: Ubiquitous occurrence of a dimethylsulfoniopropionate ABC transporter in abundant marine bacteria Article Snippet: The related genes were then cloned into the Nde I/ Xho I restriction sites of the pET-22b vector (Novagen, Germany) with a C-terminal His tag. Article Title: TERRA R-loops connect and protect sister telomeres in mitosis Article Snippet: For expression in E. coli, C19orf43 was cloned into the pET-22b Vector (Novagen) with a C-terminal His tag and expressed without the pelB leader sequence. Article Title: Ligand discovery for T cell receptors Article Snippet: The α and β chains of Ob.1A12 and Ob.2F3 TCRs were separately cloned into the Article Title: TERRA R-loops connect and protect sister telomeres in mitosis. Article Snippet: For expression in E. coli, C19orf43 was cloned into the pET-22b Vector (Novagen) with a C-terminal His tag and expressed without the pelB leader sequence. Article Title: Crystal structure and biochemical analysis suggest that YjoB ATPase is a substrate-specific molecular chaperone Article Snippet: To prepare GroEL, DNA encoding B. subtilis GroEL (residues 1-544) was inserted into the Article Title: SAR92 clade bacteria are potentially important DMSP degraders and sources of climate-active gases in marine environments Article Snippet: The amplified genes were digested and cloned into the Nde I/ Xho I restriction sites of the pET-22b vector (Novagen, Germany) to incorporate a C-terminal His tag. Article Title: Periplasmic Expression of a Novel Human Bone Morphogenetic Protein-7 Mutant in Escherichia coli Article Snippet: The fragment encoding the genetically modified mature part was successfully cloned into the NdeI/EcoRІ site of pET-22b vector (Novagen, USA). Genetically Modified:Article Title: Characterization of a Novel Esterase Est33 From an Antarctic Bacterium: A Representative of a New Esterase Family Article Snippet: The purified PCR product was ligated into the pET-22b vector (Novagen, United States) with Hind III and Nde I using NovoRec® plus One-step PCR Cloning Kit (Novoprotein, China). Article Title: Ubiquitous occurrence of a dimethylsulfoniopropionate ABC transporter in abundant marine bacteria Article Snippet: The related genes were then cloned into the Nde I/ Xho I restriction sites of the pET-22b vector (Novagen, Germany) with a C-terminal His tag. Article Title: TERRA R-loops connect and protect sister telomeres in mitosis Article Snippet: For expression in E. coli, C19orf43 was cloned into the pET-22b Vector (Novagen) with a C-terminal His tag and expressed without the pelB leader sequence. Article Title: Ligand discovery for T cell receptors Article Snippet: The α and β chains of Ob.1A12 and Ob.2F3 TCRs were separately cloned into the Article Title: TERRA R-loops connect and protect sister telomeres in mitosis. Article Snippet: For expression in E. coli, C19orf43 was cloned into the pET-22b Vector (Novagen) with a C-terminal His tag and expressed without the pelB leader sequence. Article Title: Crystal structure and biochemical analysis suggest that YjoB ATPase is a substrate-specific molecular chaperone Article Snippet: To prepare GroEL, DNA encoding B. subtilis GroEL (residues 1-544) was inserted into the Article Title: SAR92 clade bacteria are potentially important DMSP degraders and sources of climate-active gases in marine environments Article Snippet: The amplified genes were digested and cloned into the Nde I/ Xho I restriction sites of the pET-22b vector (Novagen, Germany) to incorporate a C-terminal His tag. Article Title: Periplasmic Expression of a Novel Human Bone Morphogenetic Protein-7 Mutant in Escherichia coli Article Snippet: The fragment encoding the genetically modified mature part was successfully cloned into the NdeI/EcoRІ site of pET-22b vector (Novagen, USA). Purification:Article Title: Characterization of a Novel Esterase Est33 From an Antarctic Bacterium: A Representative of a New Esterase Family Article Snippet: The purified PCR product was ligated into the pET-22b vector (Novagen, United States) with Hind III and Nde I using NovoRec® plus One-step PCR Cloning Kit (Novoprotein, China). Article Title: Ubiquitous occurrence of a dimethylsulfoniopropionate ABC transporter in abundant marine bacteria Article Snippet: The related genes were then cloned into the Nde I/ Xho I restriction sites of the pET-22b vector (Novagen, Germany) with a C-terminal His tag. Article Title: TERRA R-loops connect and protect sister telomeres in mitosis Article Snippet: For expression in E. coli, C19orf43 was cloned into the pET-22b Vector (Novagen) with a C-terminal His tag and expressed without the pelB leader sequence. Article Title: Ligand discovery for T cell receptors Article Snippet: The α and β chains of Ob.1A12 and Ob.2F3 TCRs were separately cloned into the Article Title: TERRA R-loops connect and protect sister telomeres in mitosis. Article Snippet: For expression in E. coli, C19orf43 was cloned into the pET-22b Vector (Novagen) with a C-terminal His tag and expressed without the pelB leader sequence. Article Title: Crystal structure and biochemical analysis suggest that YjoB ATPase is a substrate-specific molecular chaperone Article Snippet: To prepare GroEL, DNA encoding B. subtilis GroEL (residues 1-544) was inserted into the Article Title: SAR92 clade bacteria are potentially important DMSP degraders and sources of climate-active gases in marine environments Article Snippet: The amplified genes were digested and cloned into the Nde I/ Xho I restriction sites of the pET-22b vector (Novagen, Germany) to incorporate a C-terminal His tag. Article Title: Periplasmic Expression of a Novel Human Bone Morphogenetic Protein-7 Mutant in Escherichia coli Article Snippet: The fragment encoding the genetically modified mature part was successfully cloned into the NdeI/EcoRІ site of pET-22b vector (Novagen, USA). Polymerase Chain Reaction:Article Title: Characterization of a Novel Esterase Est33 From an Antarctic Bacterium: A Representative of a New Esterase Family Article Snippet: The purified PCR product was ligated into the pET-22b vector (Novagen, United States) with Hind III and Nde I using NovoRec® plus One-step PCR Cloning Kit (Novoprotein, China). Article Title: Ubiquitous occurrence of a dimethylsulfoniopropionate ABC transporter in abundant marine bacteria Article Snippet: The related genes were then cloned into the Nde I/ Xho I restriction sites of the pET-22b vector (Novagen, Germany) with a C-terminal His tag. Article Title: TERRA R-loops connect and protect sister telomeres in mitosis Article Snippet: For expression in E. coli, C19orf43 was cloned into the pET-22b Vector (Novagen) with a C-terminal His tag and expressed without the pelB leader sequence. Article Title: Ligand discovery for T cell receptors Article Snippet: The α and β chains of Ob.1A12 and Ob.2F3 TCRs were separately cloned into the Article Title: TERRA R-loops connect and protect sister telomeres in mitosis. Article Snippet: For expression in E. coli, C19orf43 was cloned into the pET-22b Vector (Novagen) with a C-terminal His tag and expressed without the pelB leader sequence. Article Title: Crystal structure and biochemical analysis suggest that YjoB ATPase is a substrate-specific molecular chaperone Article Snippet: To prepare GroEL, DNA encoding B. subtilis GroEL (residues 1-544) was inserted into the Article Title: SAR92 clade bacteria are potentially important DMSP degraders and sources of climate-active gases in marine environments Article Snippet: The amplified genes were digested and cloned into the Nde I/ Xho I restriction sites of the pET-22b vector (Novagen, Germany) to incorporate a C-terminal His tag. Article Title: Periplasmic Expression of a Novel Human Bone Morphogenetic Protein-7 Mutant in Escherichia coli Article Snippet: The fragment encoding the genetically modified mature part was successfully cloned into the NdeI/EcoRІ site of pET-22b vector (Novagen, USA). |